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Malformations in rat fetuses induced by trypan blue
1National Institute of Hygienic Sciences, Osaka Branch, Japan.
Jikken Dobutsu. Experimental Animals
|July 1, 1987
Summary
Trypan blue exposure during pregnancy caused fetal malformations in Wistar rats. Internal examination is crucial for detecting these developmental abnormalities, more so than skeletal analysis.
Area of Science:
- Developmental Biology
- Toxicology
- Teratology
Background:
- Teratogens can cause developmental abnormalities in fetuses.
- Wistar rats are a common model for studying teratogenicity.
- Trypan blue is a dye that has been used to induce malformations.
Purpose of the Study:
- To investigate the types and frequencies of malformations induced by trypan blue in Wistar rat fetuses.
- To compare the diagnostic value of external, skeletal, and internal examinations in detecting these malformations.
Main Methods:
- Wistar rat dams were treated with trypan blue during gestation.
- Fetuses were examined on day 20 of gestation for external, skeletal, and internal malformations.
Main Results:
- 127 fetuses exhibited abnormalities. External malformations (108 fetuses) included tail anomalies, spina bifida, club foot, exencephaly, and anal atresia. Skeletal malformations (48 fetuses) primarily involved vertebral deformities. Internal malformations (27 fetuses) included cardiac anomalies, hydrocephaly, and anophthalmia.
- 90% of fetuses with skeletal malformations also had external malformations.
- 48% of fetuses with internal malformations also had external malformations.
Conclusions:
- Trypan blue induces a range of external, skeletal, and internal malformations in Wistar rat fetuses.
- Internal examination is more effective than skeletal examination for detecting trypan blue-induced fetal malformations.