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Related Experiment Videos

Chromatin solubilization in rapidly growing hepatomas.

R I Shah1, M A Lea

  • 1Department of Biochemistry, UMDNJ-New Jersey Medical School, Newark 07103.

Experimental and Molecular Pathology
|December 1, 1987
PubMed
Summary

Rapidly growing rat hepatomas contain significant cytosolic DNA, primarily nucleosome monomers. Despite chromatin solubilization, these tumors show lower nuclear DNA endonuclease activity, possibly linked to necrosis and cation influx.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Substantial amounts of DNA have been observed in cytosol fractions of rapidly growing transplanted rat hepatomas.
  • Understanding the nature and implications of this cytosolic DNA is crucial for cancer research.

Purpose of the Study:

  • To characterize the DNA present in the cytosol of Morris hepatomas.
  • To investigate the potential DNA endonuclease activity in tumor cell nuclei.

Main Methods:

  • Preparation of cytosol fractions from transplanted rat hepatomas.
  • Agarose gel electrophoresis to analyze DNA structure.
  • Assay of DNA endonuclease activity in isolated nuclei.

Main Results:

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  • Cytosol fractions from Morris hepatomas (7288CTC, 7777, 7800) contained significant amounts of DNA.
  • Electrophoresis revealed this DNA primarily consists of nucleosome monomers, with some dimers and trimers.
  • Nuclei from rapidly growing hepatomas exhibited lower DNA endonuclease activity compared to slower-growing tumors or host livers.

    • Conclusions:

    • Rapidly growing hepatomas release nucleosome-sized DNA into the cytosol.
    • Reduced nuclear endonuclease activity in fast-growing tumors may contribute to DNA presence.
    • Focal necrosis in solid tumors could involve cation influx and subsequent DNA endonuclease activation.