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The ectodermal control of mesodermal patterns of differentiation in the developing chick wing.

J S Geduspan1, J A MacCabe

  • 1Department of Zoology, University of Tennessee, Knoxville 37996.

Developmental Biology
|December 1, 1987
PubMed
Summary
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Limb ectoderm influences chick wing development. Until stage 15, mesoderm controls dorso-ventral patterns, after which ectoderm gains this control, affecting muscle, skeletal, and feather patterns.

Area of Science:

  • Developmental Biology
  • Embryology
  • Chick Wing Development

Background:

  • Understanding the molecular and cellular mechanisms guiding embryonic development is crucial.
  • The establishment of dorso-ventral (d-v) polarity in limbs is a key developmental process.
  • The relative contributions of ectoderm and mesoderm to d-v patterning remain an area of investigation.

Purpose of the Study:

  • To investigate the influence of limb ectoderm on the dorso-ventral muscle and skeletal patterns in the developing chick wing.
  • To determine the developmental stage at which ectoderm gains control over d-v polarity.

Main Methods:

  • Recombination of stage 14-21 chick limb mesoderm and ectoderm in reversed dorso-ventral orientation.
  • Grafting of recombinants to the flank of host embryos for 10 days of development.

Related Experiment Videos

  • Analysis of muscle, skeletal, and feather patterns in the resulting wings.

    • Main Results:

    • Wings from stage 15-21 donors showed distal d-v polarity matching the reversed ectoderm and proximal polarity matching the mesoderm.
    • Ectodermal influence resulted in bidorsal feather patterns and significant d-v reversal of muscle and skeletal patterns.
    • Wings from donors younger than stage 15 exhibited d-v patterns conforming to the mesoderm.

    Conclusions:

    • Limb mesoderm controls d-v polarity until approximately stage 15 in chick wing development.
    • At stage 15, the ectoderm acquires the ability to impose its d-v information onto the mesoderm.
    • This study elucidates a critical transition in the control of embryonic limb patterning.