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Construction of Covalent Organic Framework Capsule-Based Nanoreactor for Sensitive Glucose Detection
Chao Zhong1, Guorong Li1, Wenchang Tian1
1Ministry of Education Key Laboratory of Analytical Science for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108 China.
ACS Applied Materials & Interfaces
|February 14, 2023
Summary
Researchers developed a new method to immobilize enzymes in hollow covalent organic framework (COF) capsules, enhancing enzyme activity and stability for applications like glucose detection.
Area of Science:
- Materials Science
- Biotechnology
- Nanotechnology
Background:
- Enzyme immobilization is crucial for expanding enzyme applications.
- Covalent organic frameworks (COFs) offer promising porous structures for enzyme hosting.
- Existing COF immobilization methods face challenges in maintaining high enzyme activity.
Purpose of the Study:
- To develop a versatile approach for enzyme immobilization within hollow COF capsules.
- To enhance enzyme activity, stability, and mass transfer using COF encapsulation.
- To demonstrate the construction of enzyme cascade systems within COF capsules.
Main Methods:
- Enzymes were encapsulated within hollow COF capsules using metal-organic frameworks as sacrificial templates.
- The resulting enzyme@COF structures were characterized for their porous properties and enzyme protection.
- A glucose oxidase (GOx) and cytochrome c (Cyt c) cascade system was constructed within COF capsules.
Main Results:
- The hollow COF capsules facilitated efficient mass transfer and protected encapsulated enzymes.
- Encapsulated enzymes exhibited enhanced activity and stability compared to free enzymes.
- The GOx-Cyt c@COF cascade system showed a 1.6-fold improvement in catalytic activity and was used for glucose determination.
Conclusions:
- The developed method provides an effective strategy for enzyme immobilization in COFs.
- Hollow COF capsules offer a robust platform for creating stable and active enzyme systems.
- This approach enables the design of advanced enzyme cascade systems and nanoreactors.
