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The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
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Microfluidics in Assessing Platelet Function
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Platelet Functional Testing Via High-Throughput Microtiter Plate-Based Assays.

Hem Kumar Tamang1, Emily N Stringham1, Benjamin E Tourdot1,2

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This study introduces high-throughput microplate assays for comprehensive platelet function analysis. These methods enhance throughput and reduce costs for studying platelet disorders and developing antithrombotic therapies.

Keywords:
ATP secretionaggregationcalcium mobilizationdense granule

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Area of Science:

  • Biochemistry
  • Hematology
  • Assay Development

Background:

  • Platelets are crucial for hemostasis and thrombosis.
  • Conventional platelet assays are low-throughput and require specialized equipment.
  • Miniaturization and multiplexing of assays are needed for efficient platelet function analysis.

Purpose of the Study:

  • To describe optimized, high-throughput microplate assays for comprehensive platelet functionality assessment.
  • To increase throughput and reduce costs in studying platelet biology.
  • To provide adaptable assays for drug screening and mechanism deciphering.

Main Methods:

  • Developed a panel of high-throughput microplate assays.
  • Utilized standard laboratory equipment and a plate reader.
  • Assays measure light transmission aggregation, dense-granule secretion (ATP-dependent luminescence), and cytosolic calcium levels.

Main Results:

  • Optimized and validated assays for comprehensive platelet assessment.
  • Demonstrated ability to measure multiple platelet functions (aggregation, secretion, calcium flux).
  • Assays are adaptable for independent or combined use, increasing efficiency.

Conclusions:

  • High-throughput microplate assays offer a cost-effective and efficient method for studying platelet function.
  • These assays can be used to diagnose platelet disorders, develop antithrombotic therapies, and screen for novel inhibitors.
  • The adaptable nature of these assays facilitates deciphering platelet activation mechanisms.