Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

7.6K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.6K
Ribosome Profiling02:24

Ribosome Profiling

3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proteomic insights into larval stages of coffee leaf miner (Leucoptera coffeella): A step towards the development of pest management strategies.

Comparative biochemistry and physiology. Part D, Genomics & proteomics·2026
Same author

A Novel Ocellatin-P1 Isoform from <i>Leptodactylus labyrinthicus</i> Frog Skin Secretion: Purification, Biological Properties and Three-Dimensional Structure.

International journal of molecular sciences·2026
Same author

Capturing Cardiomyocyte Cell-to-Cell Heterogeneity via Shotgun Single Cell Top-Down Proteomics.

bioRxiv : the preprint server for biology·2026
Same author

Bioconversion of Organosolv Lignin by Rumen Bacterium: Isolation, Characterization and Metabolic Profiling.

Molecules (Basel, Switzerland)·2026
Same author

Transmutation learning: Breaking the reference database barrier to unlock single-cell proteomics.

Journal of proteomics·2026
Same author

Aluminum-induced proteomic responses in Qualea dichotoma (Mart.) warm: a dataset descriptive analysis.

Scientific reports·2026

Related Experiment Video

Updated: Aug 10, 2025

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

9.5K

Revealing Corynebacterium glutamicum proteoforms through top-down proteomics.

Reynaldo Magalhães Melo1, Jaques Miranda Ferreira de Souza1, Thomas Christopher Rhys Williams2

  • 1Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil.

Scientific Reports
|February 14, 2023
PubMed
Summary
This summary is machine-generated.

This study reveals extensive post-translational modifications (PTMs) in Corynebacterium glutamicum using top-down proteomics. These PTMs on numerous proteoforms, including those in amino acid production, suggest novel regulatory roles for this industrial bacterium.

More Related Videos

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
09:26

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

Published on: May 23, 2021

3.2K
A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease
09:52

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

Published on: January 10, 2025

691

Related Experiment Videos

Last Updated: Aug 10, 2025

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

9.5K
Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
09:26

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

Published on: May 23, 2021

3.2K
A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease
09:52

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

Published on: January 10, 2025

691

Area of Science:

  • Microbiology
  • Proteomics
  • Biotechnology

Background:

  • Corynebacterium glutamicum is a key industrial microorganism for producing amino acids and other valuable compounds.
  • Post-translational modifications (PTMs) are known to regulate C. glutamicum metabolism, but comprehensive proteoform characterization was lacking.

Purpose of the Study:

  • To characterize C. glutamicum proteoforms and their post-translational modifications (PTMs) using a top-down proteomics approach.
  • To identify novel PTMs and proteoforms impacting biotechnologically relevant processes in C. glutamicum.

Main Methods:

  • Top-down mass spectrometry-based proteomics was employed for comprehensive proteoform analysis.
  • Identification and characterization of mass shifts indicative of PTMs across the C. glutamicum proteome.

Main Results:

  • 1125 proteoforms from 273 proteins were identified, with 60% exhibiting mass shifts suggesting PTMs.
  • Detected PTMs include acetylation, oxidation, and formylation.
  • Proteins involved in amino acid production, secretion, and oxidative stress showed mass shifts, including cleaved and formylated membrane proteins (mepB, SecG) and modified central metabolism proteins (OdhI).

Conclusions:

  • This is the first top-down proteome analysis of C. glutamicum, providing a detailed view of its proteoforms and PTMs.
  • The identified PTMs and novel proteoforms offer potential insights into regulating metabolic and biotechnological processes in this industrial bacterium.
  • Further investigation of these modified proteoforms could enhance C. glutamicum's utility in industrial applications.