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pPe Op inhibits HGC-27 cell proliferation, migration and invasion by upregulating miR-30b-5p and down-regulating the

Wenjun Xu1, Zhenjie Fu1, Yuqin Xu1

  • 1School of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.

Acta Biochimica Et Biophysica Sinica
|February 15, 2023
PubMed

Insights

A bioactive protein from Omphalia lapidescens upregulates miR-30b-5p, inhibiting gastric cancer cell growth. This microRNA targets RAB22A, reducing Rac1/Cdc42 activation and cancer cell proliferation, migration, and invasion.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Gastric cancer is a leading cause of cancer-related death globally.
  • Omphalia lapidescens contains bioactive compounds with potential anti-cancer properties.
  • MicroRNAs (miRNAs) play crucial roles in cancer development and progression.

Purpose of the Study:

  • To investigate the anti-gastric cancer effects of a bioactive protein (pPeOp) from Omphalia lapidescens.
  • To identify the specific miRNA involved in the anti-cancer mechanism of pPeOp.
  • To elucidate the molecular pathway through which pPeOp exerts its effects on gastric cancer cells.

Main Methods:

  • miRNA deep sequencing and verification of miR-30b-5p expression.
  • Cell proliferation, migration, and invasion assays in HGC-27 cells.
  • Immunofluorescence microscopy to assess cytoskeletal changes.
  • Bioinformatics analysis, dual-luciferase reporter assays, and Western blot to confirm target interactions.

Main Results:

  • pPeOp treatment significantly upregulated miR-30b-5p in HGC-27 cells.
  • miR-30b-5p was downregulated in clinical gastric cancer tissues.
  • pPeOp and/or miR-30b-5p inhibited HGC-27 cell proliferation, migration, and invasion.
  • miR-30b-5p was confirmed to target RAB22A, downregulating Rac1/Cdc42 expression and activation.
  • Cytoskeletal disruption and inhibition of pseudopodia formation were observed.

Conclusions:

  • miR-30b-5p mediates the anti-gastric cancer effects of pPeOp.
  • The pPeOp/miR-30b-5p axis inhibits gastric cancer progression by targeting RAB22A and disrupting the actin cytoskeleton.
  • These findings highlight miR-30b-5p as a potential therapeutic target for gastric cancer.
Keywords:
Rac1/Cdc42Schroet.gastric cancermiR-30b-5p

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