Inferring Gene Regulatory Networks and Predicting the Effect of Gene Perturbations via IQCELL
Tiam Heydari1,2, Peter W Zandstra1,2
1School of Biomedical Engineering, University of British Columbia, Vancouver, BC, Canada.
Methods in Molecular Biology (Clifton, N.J.)
|February 15, 2023
Abstract:
IQCELL is a platform that infers Boolean gene regulatory networks from single-cell RNA sequencing data. Boolean networks can be simulated under normal and perturbed conditions. In this chapter, we provide a detailed guideline for implementing IQCELL from a raw dataset. The steps include processing data, inferring informative genes, inferring gene regulatory network, and simulating the resulted network under normal and perturbed conditions.
Keywords:
Boolean GRNsCausal inferenceDevelopmental trajectoryGene perturbationsGene regulationscRNA-seqMore Related Videos
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