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Updated: Aug 10, 2025
Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors
Published on: April 13, 2019
De novo biosynthesis of 2'-fucosyllactose in engineered Pichia pastoris
Difan Qian1, Chunyue Zhang1,2, Chen Deng1,2
1State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Meilong Road 130, Shanghai, 200237, China.
Purpose:
Pichia pastoris is well known for its ability to produce short and low-immunogenic humanized glycosyl chains onto recombinant glycoproteins, it was thus speculated to be applicable to synthesize oligosaccharides. In this study, generally recognized as safe (GRAS) microorganism Pichia pastoris GS115 was tested for its potential to be used as a new synthetic chassis to produce the most abundant human milk oligosaccharide 2'-fucosyllactose (2'-FL).
Methods:
To enable the de novo synthesis of 2'-FL, lactose transporter lac12, two enzymes of gmd, gmer, and fucosyltransferases futC were integrated into the genome of P. pastoris, under the control of constitutive PGAP promoter.
Results:
The resulting recombinant yeasts yielded up to 0.276 g/L through culture optimization in a 5 L bioreactor.
Conclusion:
To our knowledge, this is the first report of 2'-FL production in engineered Pichia pastoris. This work is a good starting point to produce 2'-FL using Pichia pastoris as a viable chassis.
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