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Establishing protein expression profiles involved in tooth development using a proteomic approach.

Junko Shimomura-Kuroki1, Masayuki Tsuneki2,3,4, Hiroko Ida-Yonemochi3

  • 1Department of Pediatric Dentistry, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamauracho, Chuo-Ku, Niigata, 951-8580, Japan. jshimo@ngt.ndu.ac.jp.

Odontology
|February 15, 2023
PubMed
Summary

This proteomic study reveals distinct roles for ATP synthase subunit beta (ATP5B), receptor of activated protein C kinase 1 (RACK1), and calreticulin (CALR) during tooth development and amelogenesis.

Keywords:
MouseOrganogenesisProteinProteomicsTooth

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Area of Science:

  • Developmental Biology
  • Proteomics
  • Biochemistry

Background:

  • Tooth development involves complex gene regulation, but protein dynamics may not directly mirror mRNA expression.
  • Understanding protein expression is crucial for deciphering developmental abnormalities.

Purpose of the Study:

  • To comprehensively analyze protein expression in tooth germ tissues during development using a proteomic approach.
  • To investigate the specific roles and expression patterns of key proteins (ATP5B, RACK1, CALR) during amelogenesis.

Main Methods:

  • Laser microdissection of epithelial and mesenchymal tissues from mouse molar tooth germs.
  • Mass spectrometry for comprehensive protein analysis.
  • Immunohistochemical analysis of ATP5B, RACK1, and CALR expression during amelogenesis in mouse incisors.

Main Results:

  • Identified ATP5B, RACK1, and CALR as highly expressed proteins.
  • RACK1 showed specific expression during proliferation and differentiation stages.
  • ATP5B and CALR expression intensified during maturation and pigmentation stages, with distinct timing.

Conclusions:

  • RACK1 is vital for maintaining cell proliferation and differentiation in incisor development.
  • ATP5B and CALR are involved in mineral transport, organic material removal, and matrix deposition during tooth formation.