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Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates
Published on: July 22, 2019
Type VI Secretion Systems: Environmental and Intra-host Competition of Vibrio cholerae
Francis Santoriello1,2, Stefan Pukatzki3
1Department of Biology, The City College of New York, New York, NY, USA.
Abstract:
The Vibrio Type VI Secretion System (T6SS) is a harpoon-like nanomachine that serves as a defense system and is encoded by approximately 25% of all gram-negative bacteria. In this chapter, we describe the structure of the T6SS in different Vibrio species and outline how the use of different T6SS effector and immunity proteins control kin selection. We summarize the genetic loci that encode the structural elements that make up the Vibrio T6SSs and how these gene clusters are regulated. Finally, we provide insights into T6SS-based competitive dynamics, the role of T6SS genetic exchange in those competitive dynamics, and roles for the Vibrio T6SS in virulence.
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