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Purification of Functional Gene Transfer Agents Using Two-Step Preparative Monolithic Chromatography
Evan Langille1,2, Christina S Bottaro1, Andrew S Lang2
1Department of Chemistry and Memorial University of Newfoundland, St. John's, Canada.
Background:
Gene transfer agents (GTAs) are phage-like particles that transfer cellular genomic DNA between cells. A hurdle faced in studying GTA function and interactions with cells is the difficulty in obtaining pure and functional GTAs from cultures.
Materials And Methods:
We used a novel two-step method for purification of GTAs from R. capsulatus by monolithic chromatography.
Results:
Our efficient and simple process had advantages compared to previous approaches. The purified GTAs retained gene transfer activity and the packaged DNA could be used for further studies.
Conclusions:
This method is applicable to GTAs produced by other species and small phages, and could be useful for therapeutic applications.
