Related Experiment Video
Updated: Aug 9, 2025
10:17
High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
9.7K
An overview of recent progress in multiple dual-mode counter-current chromatography
Fu-Xin Zhang1,2, Jules Muhire1,2, Xiao Sun1,2
1CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory of Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, China.
Journal of Separation Science
|February 16, 2023
Summary
Multiple dual-mode elution enhances counter-current chromatography (CCC) separation efficiency by dynamically switching between normal and reverse elution modes. This technique offers improved purification for complex samples.
Area of Science:
- Chromatography
- Separation Science
- Analytical Chemistry
Background:
- Counter-current chromatography (CCC) is an advanced separation and purification technique.
- Elution modes are critical for optimizing CCC performance.
- Multiple dual-mode elution (MDME) is a novel CCC elution strategy.
Purpose of the Study:
- To review the development, applications, and characteristics of multiple dual-mode elution in CCC.
- To highlight the advantages and limitations of MDME for complex sample separation.
- To discuss the future outlook of this advanced chromatographic technique.
Main Methods:
- Reviewing recent literature on multiple dual-mode elution in counter-current chromatography.
- Analyzing the principles of switching between normal and reverse elution modes.
- Summarizing the application of MDME in separating complex mixtures.
Main Results:
- MDME significantly improves separation efficiency by leveraging the liquid nature of CCC phases.
- This technique effectively enhances the purification of complex samples.
- MDME offers a dynamic approach to chromatographic separations.
Conclusions:
- Multiple dual-mode elution represents a significant advancement in counter-current chromatography.
- Its ability to enhance separation efficiency makes it highly valuable for complex sample analysis.
- Further research and application development are anticipated for MDME.
Related Concept Videos
01:15High-Performance Liquid Chromatography: Types of Detectors
682
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
682
01:11High-Performance Liquid Chromatography: Introduction
2.2K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
2.2K
01:27Controlled-Current Coulometry: Overview
268
Controlled current coulometry, also known as amperostatic coulometry, is a technique used in electrochemical analysis to measure the quantity of a substance through the controlled passage of current. It involves the application of a constant current to an electrochemical cell containing the analyte of interest. As the current flows through the cell, the analyte undergoes a redox reaction at the electrode surface, resulting in a charge transfer. By monitoring the time required for a certain...
268
00:57High-Performance Liquid Chromatography: Instrumentation
2.0K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
2.0K
01:05High-Performance Liquid Chromatography: Elution Process
571
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
571
01:10Chromatography: Introduction
4.5K
Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
4.5K