Related Experiment Video
Updated: Aug 9, 2025
09:03
Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay
Published on: June 30, 2023
1.0K
Transcription factors regulating vasculogenesis and angiogenesis
Sophie Payne1, Alice Neal1, Sarah De Val1
1Department of Physiology, Anatomy and Genetics, Institute of Developmental and Regenerative Medicine, University of Oxford, Oxford, UK.
Summary
Transcription factors (TFs) regulate endothelial cell (EC) development and differentiation. ECs use combinations of TFs, not a single master regulator, to achieve precise gene expression patterns during vascular network formation.
Area of Science:
- Developmental biology
- Molecular biology
- Cell biology
Background:
- Transcription factors (TFs) are critical for gene expression regulation in endothelial cells (ECs).
- Endothelial cells exhibit heterogeneity essential for vascular network patterning and angiogenesis.
- ECs lack a single master regulator, relying on combinatorial TF activity.
Purpose of the Study:
- To review the roles of TFs in mammalian vasculogenesis and angiogenesis.
- To highlight the importance of TF combinations in EC development and specialization.
Main Methods:
- Literature review of TFs involved in EC development.
- Analysis of TF roles in vasculogenesis and angiogenesis.
Main Results:
- Discusses the cohort of TFs governing gene expression during EC development.
- Emphasizes the combinatorial action of TFs for precise spatial and temporal gene regulation.
- Highlights TF involvement in hierarchical vascular network patterning and angiogenic growth.
Conclusions:
- Combinatorial TF activity is essential for EC heterogeneity and function.
- Understanding TF networks is key to deciphering vascular development and disease.
Related Concept Videos
01:24Regulation of Angiogenesis and Blood Supply
2.7K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.7K
01:10Mechanism of Angiogenesis
5.7K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
5.7K
02:16Transcription Factors
76.3K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
76.3K
01:30General Transcription Factors
5.4K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.4K
02:23Master Transcription Regulators
7.0K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.0K
01:07Development of Blood Vessels
680
The development of the vascular system in a fetus is a complex and intricate process that begins as early as 15 to 16 days post-conception. This process starts outside the embryo, specifically in the mesoderm of the yolk sac, chorion, and connecting stalk. Approximately two days later, the formation of blood vessels occurs within the embryo itself.
The initial formation of this system is facilitated by the small amount of yolk present in the ovum and yolk sac. Blood vessels originate from...
The initial formation of this system is facilitated by the small amount of yolk present in the ovum and yolk sac. Blood vessels originate from...
680