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Related Experiment Videos

Complement-dependent hemodynamic and hematologic changes in the rabbit.

R J Ulevitch, C G Cochrane

    Inflammation
    |September 1, 1977
    PubMed
    Summary
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    Cobra factor (CoF) injection causes rapid, reversible drops in blood pressure and platelets, dependent on complement C3. Histamine H2-receptor blockade prevents these CoF-induced effects.

    Area of Science:

    • Immunology
    • Pharmacology
    • Physiology

    Background:

    • Cobra factor (CoF) is an anticomplementary protein that activates the complement system.
    • The complement system plays a crucial role in immune responses and inflammation.
    • Endotoxins from bacteria can trigger significant physiological responses.

    Purpose of the Study:

    • To investigate the effects of cobra factor (CoF) on blood pressure and platelets in rabbits.
    • To determine the role of complement component C3 in CoF-induced changes.
    • To compare the effects of CoF with those of *Serratia marcescens* endotoxin.

    Main Methods:

    • Intravenous injection of CoF and *S. marcescens* endotoxin in rabbits.
    • Measurement of mean arterial blood pressure and circulating platelet counts.

    Related Experiment Videos

  • Utilizing rabbits genetically deficient in complement component C6.
  • Employing C3 depletion strategies.
  • Administering the histamine H2-receptor antagonist burimamide.

    • Main Results:

    • CoF injection caused rapid, reversible decreases in blood pressure and platelets, dependent on complement C3.
    • These CoF-induced effects were blocked by burimamide, suggesting a histamine-mediated mechanism.
    • *S. marcescens* endotoxin induced acute, C3-dependent changes in blood pressure and platelets.
    • C3 depletion did not affect the prolonged hypotensive and platelet-lowering effects or lethality of endotoxin.

    Conclusions:

    • Complement C3 is essential for the acute hypotensive and thrombocytopenic effects of CoF.
    • Histamine release, mediated via H2 receptors, is involved in the CoF-induced response.
    • The acute effects of *S. marcescens* endotoxin are C3-dependent, but later responses and lethality are not.