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Metabolomic and transcriptomic profiles after immune stimulation in the zebrafish testes
T A van Gelderen1, C Ladisa2, M Salazar-Moscoso1
1Institut de Ciències del Mar - Consejo Superior de Investigaciones Científicas (ICM-CSIC), Department of Renewable Marine Resources, 08003 Barcelona, Spain.
Genomics
|February 16, 2023
Summary
This study reveals how immune challenges affect zebrafish testes, identifying key metabolites and genes involved in reproduction and immunity. Findings offer insights for breeding more disease-resistant fish in aquaculture.
Area of Science:
- Aquaculture
- Fish immunology
- Reproductive biology
Background:
- High-density fish farming increases disease susceptibility and stress, impacting fish health and productivity.
- Understanding molecular responses in fish gonads to immune challenges is crucial for improving aquaculture practices.
Purpose of the Study:
- To investigate the metabolomic and transcriptomic changes in zebrafish testes following an induced immune response.
- To elucidate the molecular mechanisms linking immune challenges to reproductive systems in fish.
Main Methods:
- Zebrafish testes were subjected to an immune challenge for 48 hours.
- Metabolome profiling was performed using ultra-high-performance liquid chromatography (LC-MS).
- Transcriptome profiling was conducted using RNA-sequencing (RNA-seq).
Main Results:
- 20 distinct metabolites and 80 differentially expressed genes were identified.
- Glutamine and succinic acid were the most abundant released metabolites.
- 27.5% of identified genes were related to immune or reproductive systems.
- Pathway analysis revealed crosstalk between genes (cad, iars) and succinate metabolite.
Conclusions:
- The study deciphers complex interactions between the reproductive and immune systems in fish under stress.
- Findings provide a foundation for developing strategies to enhance broodstock resistance to diseases.
- This research can contribute to improving aquaculture sustainability and fish health management.
