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Recent advances and applications of CRISPR-Cas9 in cancer immunotherapy

Zaoqu Liu1,2,3, Meixin Shi1, Yuqing Ren4

  • 1Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.

Molecular Cancer
|February 16, 2023
PubMed

Insights

CRISPR-Cas9 gene editing offers a powerful new approach to enhance cancer immunotherapy by overcoming current limitations and improving treatment outcomes. This technology enables precise genetic modifications for better cancer therapies.

Area of Science:

  • Oncology
  • Genetics
  • Immunology

Background:

  • Cancer remains a leading global health challenge with limited therapeutic options.
  • Immunotherapy has advanced cancer treatment but faces limitations and adverse effects.
  • Gene editing technologies, particularly CRISPR-Cas9, present novel strategies for cancer therapy.

Purpose of the Study:

  • To review the mechanism and applications of CRISPR-Cas9 in cancer immunotherapy.
  • To explore the impact of CRISPR-induced double-strand breaks on immunotherapy efficacy.
  • To discuss the role of CRISPR-Cas9 in target identification and future clinical translation.

Main Methods:

  • Review of CRISPR-Cas9 genome editing mechanisms.
  • Analysis of CRISPR-Cas9 applications in cancer immunotherapy (knockout/knockin).
  • Discussion of CRISPR-Cas9-based screening and spatial genomics.

Main Results:

  • CRISPR-Cas9 facilitates precise gene manipulation for immunotherapy enhancement.
  • CRISPR-induced double-strand breaks can modulate anti-tumor immune responses.
  • Genome-wide screening with CRISPR-Cas9 aids in identifying novel therapeutic targets.

Conclusions:

  • CRISPR-Cas9 technology holds significant potential to overcome limitations in cancer immunotherapy.
  • Further research and clinical translation are crucial for realizing the full impact of CRISPR-Cas9 in oncology.
  • This technology promises advancements in basic research, translational medicine, and clinical applications for cancer treatment.
Keywords:
CRISPR-Cas9Cancer immunotherapyCellular therapyMechanismNK/macrophage

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