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Related Experiment Video

Updated: Aug 9, 2025

Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging
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HiExM: high-throughput expansion microscopy enables scalable super-resolution imaging.

John H Day1, Catherine Marin Della Santina1, Pema Maretich2

  • 1Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 USA.

Biorxiv : the Preprint Server for Biology
|February 17, 2023
PubMed
Summary

High-throughput Expansion Microscopy (HiExM) enables scalable nanoscale imaging of cells in 96-well plates. This method improves detection of cellular phenotypes, such as drug-induced DNA changes in cardiomyocytes.

Keywords:
expansion microscopyhigh-throughputnanoscopysuper-resolution imaging

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Area of Science:

  • Cell Biology
  • Microscopy Techniques
  • Biotechnology

Background:

  • Expansion microscopy (ExM) allows nanoscale imaging with standard microscopes by expanding fixed samples.
  • Current ExM methods are limited in throughput, restricting simultaneous sample processing.

Purpose of the Study:

  • To develop a high-throughput platform for expansion microscopy (HiExM).
  • To enable scalable nanoscale imaging of cells in standard 96-well plates.
  • To improve the ease and scalability of image acquisition for ExM.

Main Methods:

  • Developed High-throughput Expansion Microscopy (HiExM) for cells in 96-well plates.
  • Achieved approximately 4.2x isotropic expansion of cells within wells and across plates.
  • Integrated HiExM with high-throughput confocal imaging platforms.

Main Results:

  • Demonstrated successful expansion and imaging of cells in a 96-well plate format.
  • Showed improved detection of doxorubicin's dose-dependent effects on cardiomyocyte nuclear DNA compared to unexpanded samples.
  • Validated HiExM's scalability and robustness for biological research.

Conclusions:

  • HiExM significantly enhances the throughput of expansion microscopy.
  • The platform enables scalable super-resolution imaging for drug screening and cellular phenotype analysis.
  • HiExM broadens the application of ExM in biological research by improving scalability and ease of use.