Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

52.6K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.6K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

99
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
99
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.2K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.2K
Homologous Recombination02:31

Homologous Recombination

50.8K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.8K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

76
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
76

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transmission Electron Microscopy for Structural Insights into Bacterial Cellulose Nanowhiskers in Ternary Deep Eutectic Solvent.

ACS measurement science au·2026
Same author

Polyvalent Guide RNAs Enhance the CRISPR-Mediated Suppression of a Human Coronavirus.

ACS synthetic biology·2026
Same author

Assessing Knowledge Distillation of a Multi-Emitter Localizing Neural Network for Applications in Stochastic Optical Reconstruction Microscopy.

bioRxiv : the preprint server for biology·2026
Same author

Exploring Carbon Dot Nanoparticles for Imaging and Cellular Interaction in Triple-Negative Breast Cancer.

International journal of nanomedicine·2025
Same author

Developing a Modular Platform for the Detection of microRNAs Using Rolling Circle Amplification and Multi-Primed Chain Amplification.

Journal of molecular recognition : JMR·2025
Same author

Systematic Optimization Enables Near-Perfect In Vitro Transformation Efficiencies for <i>Spirodela polyrhiza</i> (Greater Duckweed).

bioRxiv : the preprint server for biology·2025
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026
Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Aug 9, 2025

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

1.3K

Digital data storage on DNA tape using CRISPR base editors.

Afsaneh Sadremomtaz, Robert F Glass, Jorge Eduardo Guerrero

    Biorxiv : the Preprint Server for Biology
    |February 17, 2023
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed DNA Mutational Overwriting Storage (DMOS) for eco-friendly digital data archiving. This novel system uses CRISPR base editing to write and recover data on DNA tapes, overcoming limitations of current DNA synthesis methods.

    More Related Videos

    Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
    09:22

    Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

    Published on: February 28, 2021

    5.5K
    Genome Editing in Mammalian Cell Lines using CRISPR-Cas
    07:56

    Genome Editing in Mammalian Cell Lines using CRISPR-Cas

    Published on: April 11, 2019

    21.9K

    Related Experiment Videos

    Last Updated: Aug 9, 2025

    CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
    07:49

    CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

    Published on: May 30, 2025

    1.3K
    Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
    09:22

    Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

    Published on: February 28, 2021

    5.5K
    Genome Editing in Mammalian Cell Lines using CRISPR-Cas
    07:56

    Genome Editing in Mammalian Cell Lines using CRISPR-Cas

    Published on: April 11, 2019

    21.9K

    Area of Science:

    • Biotechnology
    • Data Storage
    • Molecular Engineering

    Background:

    • Digital data storage faces physical limitations and increasing demand.
    • DNA offers superior durability, capacity, and energy efficiency for data archiving.
    • Current DNA data storage methods often rely on non-scalable, waste-producing de-novo synthesis.

    Approach:

    • Developed DNA Mutational Overwriting Storage (DMOS), a novel molecular digital data storage system.
    • Leveraged in vitro CRISPR base-editing reactions for combinatorial, addressable, orthogonal, and independent data writing.
    • Utilized greenly synthesized DNA tapes as the storage medium.

    Key Points:

    • DMOS enables data storage by inducing specific mutations on DNA tapes.
    • CRISPR base editing allows for precise and independent writing of data.
    • Successfully demonstrated data writing and accurate recovery of a logo and study title.

    Conclusions:

    • DMOS presents a scalable and environmentally friendly alternative for DNA data storage.
    • This approach overcomes the limitations of toxic waste associated with de-novo DNA synthesis.
    • The system shows promise for future archival digital memory solutions.