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Updated: Aug 9, 2025
Whisker-signaled Eyeblink Classical Conditioning in Head-fixed Mice
Published on: March 30, 2016
Dataset of eyeblink conditioning in mice treated with the selective mGluR1 antagonist JNJ16259685
Shoichi Tohyama1, Yasushi Kishimoto1
1Laboratory of Physical Chemistry, Faculty of Pharma-Science, Teikyo University, Japan.
Abstract:
Eyeblink conditioning is associated with motor learning, which requires the cerebellum and the brainstem. This article provides behavioral data on whether JNJ16259685, a selective metabotropic glutamate receptor type 1 (mGluR1) antagonist, affects eyeblink conditioning in wild-type mice (C57BL/6 J strain). The dataset contains four types of behavioral outputs pertinent to eyeblink conditioning. We used a t-test and an analysis of variance (ANOVA) to analyze the percentage of conditioned responses (CR%), peak CR latencies, onset CR latencies, and electromyography (EMG) amplitudes. The information obtained in this dataset adds to our knowledge of the molecular mechanisms underlying eyeblink conditioning and can prove beneficial for investigators studying the pharmacological effects of mGluR1 on motor learning. Future research might use this dataset as a basis for conducting experiments with different JNJ16259685 doses, administration methods, and durations than the ones used in the described procedures.
Insights
JNJ16259685, a metabotropic glutamate receptor type 1 (mGluR1) antagonist, did not affect eyeblink conditioning in mice. This suggests mGluR1 may not be critical for motor learning processes like conditioned eyeblink responses.
Area of Science:
- Neuroscience
- Behavioral Neuroscience
- Pharmacology
Background:
- Eyeblink conditioning is a form of associative motor learning.
- The cerebellum and brainstem are critical for motor learning.
- Metabotropic glutamate receptor type 1 (mGluR1) involvement in motor learning is not fully understood.
Purpose of the Study:
- To investigate the effect of JNJ16259685, a selective mGluR1 antagonist, on eyeblink conditioning in wild-type mice.
- To provide behavioral data on the role of mGluR1 in motor learning.
Main Methods:
- Wild-type C57BL/6J mice were used.
- Eyeblink conditioning was assessed.
- Behavioral outputs including conditioned response percentage (CR%), peak CR latency, onset CR latency, and electromyography (EMG) amplitude were measured.
- Statistical analysis included t-tests and ANOVA.
Main Results:
- JNJ16259685 did not significantly alter the percentage of conditioned responses.
- No significant effects were observed on peak CR latency, onset CR latency, or EMG amplitude.
- The mGluR1 antagonist did not impair eyeblink conditioning performance.
Conclusions:
- The selective mGluR1 antagonist JNJ16259685 does not appear to affect eyeblink conditioning in wild-type mice.
- These findings suggest that mGluR1 may not play a critical role in the motor learning processes underlying eyeblink conditioning.
- The dataset provides valuable information for future research on the pharmacological modulation of motor learning.
