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Smooth endoplasmic reticulum cluster presence does not affect embryo ploidy.

Yamato Mizobe1, Yukari Kuwatsuru2, Yuko Kuroki2

  • 1Takeuchi Ladies Clinic/Center for Reproductive Medicine, 502-2 Higashimochida, Aira-shi, Kagoshima, 899-5421, Japan. takeuchi.lab.1f@gmail.com.

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|February 17, 2023
PubMed
Summary

The presence of smooth endoplasmic reticulum clusters (sERC) in oocytes is linked to higher hormone levels and abnormal fertilization rates. However, sERC presence did not impact embryonic development or blastocyst ploidy, indicating no effect on euploidy rates.

Keywords:
ChildbirthNext-generation sequencingPloidy of embryosPreimplantation genetic testingSmooth endoplasmic reticulum cluster

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Area of Science:

  • Reproductive biology
  • Embryology
  • In vitro fertilization

Background:

  • The smooth endoplasmic reticulum cluster (sERC) is a cellular feature observed in oocytes.
  • Its impact on oocyte quality, embryonic development, and ploidy is not fully understood.
  • Understanding these factors is crucial for improving IVF outcomes.

Purpose of the Study:

  • To investigate the association between the presence of smooth endoplasmic reticulum clusters (sERC) and key reproductive parameters.
  • To determine the effect of sERC on embryonic development and blastocyst ploidy.
  • To evaluate hormonal profiles and fertilization outcomes in relation to sERC presence.

Main Methods:

  • Oocytes from 2019-2021 were classified based on sERC presence and normalcy.
  • Hormonal levels (estradiol, progesterone, AMH, FSH, HMG) were compared between groups.
  • Fertilization, degeneration, and abnormal fertilization rates were analyzed.
  • Blastocyst development, quality, and ploidy (via NGS) were assessed after ICSI and embryo transfer.

Main Results:

  • sERC presence correlated with significantly higher serum estradiol, progesterone, and AMH levels (P<0.01).
  • Abnormal fertilization rates were significantly higher in oocytes with sERC (16.1%) compared to normal oocytes (6.2-7.1%) (P<0.01).
  • No significant differences in blastocyst rates or ploidy were observed between sERC (+) and sERC (-) groups.

Conclusions:

  • While sERC presence is associated with altered hormonal profiles and increased abnormal fertilization, it does not appear to affect overall embryonic development or blastocyst ploidy.
  • Euploidy rates remain similar regardless of sERC presence, suggesting it may not be a detrimental factor for chromosomal normality in embryos.