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Transformation

Transformation

Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...

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Related Experiment Video

Updated: Aug 9, 2025

Author Spotlight: Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016

Author Spotlight: Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016

Published on: June 23, 2023

An optimized electrotransformation protocol for Lactobacillus jensenii.

Elsa Fristot¹, Thomas Bessede¹, Miguel Camacho Rufino¹

¹Centre de Biologie Structurale (CBS), University of Montpellier, INSERM U 1054, CNRS UMR 5048, Montpellier, France.

|February 17, 2023

Summary

This summary is machine-generated.

Researchers optimized electrotransformation for Lactobacillus jensenii, a common vaginal bacterium. This breakthrough significantly improves genetic engineering efficiency for potential gynecological health applications.

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Last Updated: Aug 9, 2025

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Area of Science:

Microbiology
Synthetic Biology
Gynecological Health

Background:

Engineered bacteria show promise for in situ disease detection and treatment.
The female genitourinary tract has pathologies like STDs and cancer that could benefit from engineered microbes.
Limited engineering of bacteria from the female genitourinary tract is due to low transformability.

Purpose of the Study:

To develop an optimized electrotransformation protocol for Lactobacillus jensenii, a key vaginal microbe.
To enhance the genetic engineering capabilities of L. jensenii for potential therapeutic applications in gynecology.

Main Methods:

Optimized electrotransformation buffers, electric field parameters, cuvette type, and DNA quantity.
Tested transformation efficiency in L. jensenii ATCC 25258.
Identified suitable plasmids for reporter gene expression.
Validated the protocol on three independent clinical isolates of L. jensenii.

Main Results:

Achieved an 80-fold improvement in transformation efficiency for L. jensenii.
Reached transformation efficiencies up to 3.5·10^3 CFUs/μg of DNA.
Confirmed plasmid maintenance and reporter gene expression.
Demonstrated increased transformability in clinical isolates.

Conclusions:

The optimized protocol significantly enhances L. jensenii transformability.
This advancement facilitates the genetic engineering of L. jensenii.
Enables the development of engineered L. jensenii for gynecological healthcare challenges.