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Screening and identification of hub-gene associated with brain metastasis in breast cancer
Xiao-Gang Li1, Chao Niu, Ping Lu
1Department of General Surgery, Affiliated Hospital of Yunnan University, Kunming, China.
Background:
The presence of breast cancer in the brain, also known as brain metastasis (BMS), is the primary reason for a bad prognosis in cases of breast cancer. Breast cancer is the most prevalent malignant tumor seen in women in developing nations. At present, there is no effective method to inhibit brain metastasis of breast cancer. Therefore, it is necessary to conduct a systematic study on BMS of breast cancer, which will not provide ideas and sites for follow-up studies on the treatment and inhibition of BMS.
Methods:
In this study, data set GSE43837 was screened from gene expression omnibus database, and then R language tool was used for differential analysis of its expression spectrum, The gene ontology functional enrichment and Kyoto encyclopedia of genes and genomes signal pathway enrichment analyses, as well as the interactive gene retrieval tool for hub-gene analysis, were performed.
Results:
According to the findings, the primary genes linked to breast cancer brain metastases are those that involve interactions between cytokines and their respective receptors and between neuroactive ligands and their respective receptors. The majority of the gene ontology enrichment took place in the extracellular structural tissues, the extracellular matrix tissues, and the second message-mediated signaling. We were able to identify 8 genes that are linked to breast cancer spreading to the brain. The gene score for matrix metallopeptidase1 (MMP-1) was the highest among them, and the genes MMP10, tumor necrosis factor alpha-inducible protein 8, collagen type I alpha 2 chain, vascular cell adhesion molecule 1, and TNF superfamily member 11 were all connected to 1 another in an interaction way.
Conclusions:
There is a possibility that the 8 key genes that were identified in this research are connected to the progression of BMS in breast cancer. Among them, MMP1 is 1 that has the potential to have a role in the diagnosis and treatment of BMS in breast cancer.
Insights
Researchers identified 8 key genes involved in breast cancer brain metastasis (BMS). Matrix metallopeptidase 1 (MMP-1) shows potential for diagnosing and treating BMS, offering new avenues for research.
Area of Science:
- Oncology
- Genomics
- Molecular Biology
Background:
- Brain metastasis (BMS) from breast cancer significantly worsens patient prognosis.
- Effective treatments to inhibit breast cancer BMS are currently lacking.
- Systematic study of BMS is crucial for developing targeted therapies.
Purpose of the Study:
- To identify key genes associated with breast cancer brain metastasis.
- To explore potential diagnostic and therapeutic targets for BMS.
Main Methods:
- Utilized the GSE43837 dataset from the Gene Expression Omnibus database.
- Performed differential gene expression analysis using R language.
- Conducted Gene Ontology and KEGG pathway enrichment analyses, along with hub-gene analysis.
Main Results:
- Identified key genes involved in cytokine-receptor and neuroactive ligand-receptor interactions.
- Gene ontology enrichment was prominent in extracellular structural/matrix tissues and second messenger signaling.
- Eight genes linked to breast cancer brain metastasis were identified, with MMP-1 showing the highest score.
Conclusions:
- The identified 8 genes may play a role in breast cancer BMS progression.
- Matrix metallopeptidase 1 (MMP-1) presents potential as a diagnostic and therapeutic target for BMS.
