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Updated: Sep 12, 2026

High-throughput Saccharification Assay for Lignocellulosic Materials
Published on: July 3, 2011
High bioethanol titer and yield from phosphoric acid plus hydrogen peroxide pretreated paper mulberry wood through
Pleasure Chisom Ajayo1, Qing Wang1, Mei Huang1
1Institute of Ecological and Environmental Sciences, College of Environmental Sciences, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China; Rural Environment Protection Engineering & Technology Center of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
Abstract:
The optimization of key simultaneous saccharification and fermentation (SSF) parameters for bioethanol production from phosphoric acid plus hydrogen peroxide pretreated paper mulberry wood was carried out under two isothermal scenarios; the yeast optimum and trade-off temperatures of 35 and 38 °C, respectively. The optimal conditions established for SSF at 35 °C (solid loading: 16%; enzyme dosage: 9.8 mg protein/g glucan; and yeast concentration: 6.5 g/L) achieved high ethanol titer and yield of 77.34 g/L and 84.60% (0.432 g/g), respectively. These corresponded to 1.2 and 1.3-folds increases, compared to the results of the optimal SSF at a relatively higher temperature of 38 °C. The information from this study would prove beneficial in reducing process energy demands to some extent, while also helping to achieve high levels of both ethanol concentration and yield that are desired in cellulosic ethanol production.
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