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Slide-to-Slide Tissue Transfer and Array Assembly From Limited Samples for Comprehensive Molecular Profiling.

Stephanie E Weissinger1, N Zeke Georgantas2, Julia C Thierauf2

  • 1Institute of Pathology, Alb Fils Clinics GmbH, Göppingen, Germany; Institute of Pathology, University Hospital Ulm, Ulm, Germany.

Laboratory Investigation; a Journal of Technical Methods and Pathology
|February 21, 2023
PubMed
Summary

A new slide-to-slide (STS) transfer technique enables tissue microarrays (TMA) construction from limited tissue samples. This method overcomes challenges in high-throughput molecular profiling, proving effective for small biopsies and rare tumors.

Keywords:
biomarkerlimitationslimited tissueorphan diseasetissue rescue

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Area of Science:

  • Biomedical Sciences
  • Translational Research
  • Molecular Pathology

Background:

  • Tissue microarrays (TMA) are crucial for high-throughput molecular profiling in translational research.
  • Limited tissue availability in small biopsies or rare tumors restricts comprehensive analysis.
  • Existing methods are often insufficient for molecular profiling of scarce tissue samples.

Purpose of the Study:

  • To develop and validate a novel tissue transfer method for constructing TMAs from limited tissue sections.
  • To enable high-throughput molecular profiling on small biopsy or rare tumor samples.
  • To present a reliable and cost-effective technique for maximizing data acquisition from minimal tissue.

Main Methods:

  • Developed a slide-to-slide (STS) transfer technique involving chemical exposure (xylene-methacrylate exchange), rehydrated lifting, and microdissection.
  • Tissue fragments (methacrylate-tissue tiles) were remounted onto recipient slides to create STS array slides.
  • Assessed technique efficacy through dropout rates, transfer efficiency, antigen retrieval, immunohistochemistry, FISH, and nucleic acid extraction yields.

Main Results:

  • The STS technique demonstrated high transfer efficacy (>93%) with minimal dropouts (0.7%-6.2%), which were successfully rescued.
  • Immunohistochemistry, fluorescent in situ hybridization (FISH), DNA, and RNA extraction yields were comparable to traditional methods.
  • The method proved effective across various antigen-retrieval techniques and stain types.

Conclusions:

  • The slide-to-slide (STS) transfer is a quick, reliable, and cost-effective method for creating tissue microarrays (TMA) from limited tissue.
  • This technique significantly expands the utility of TMAs for molecular profiling in translational research and clinical practice.
  • STS transfer allows for the generation of more molecular data from scarce tissue samples, addressing a critical limitation in pathology.