Related Experiment Video
Updated: Aug 9, 2025
09:37
A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation
Published on: March 15, 2018
13.3K
A Novel Role for RILP in Regulating Osteoclastogenesis and Bone Resorption
Biao Wu1, Jie Shang1, Shiyuan Lin1
1Department of Orthopaedics, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China; Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, Guangdong, China.
Laboratory Investigation; a Journal of Technical Methods and Pathology
|February 21, 2023
Summary
Rab interacting lysosomal protein (RILP) is crucial for osteoclast formation and function. Inhibiting RILP reduces osteoclast activity, offering potential for treating bone diseases like osteoporosis.
Area of Science:
- Cell Biology
- Bone Biology
- Molecular Biology
Background:
- Osteoporosis stems from excessive bone resorption by osteoclasts.
- Osteoclast formation and function mechanisms are not fully understood.
- Rab interacting lysosomal protein (RILP) role in osteoclasts is investigated.
Purpose of the Study:
- To investigate the role of RILP in osteoclastogenesis and bone resorption.
- To explore RILP as a potential therapeutic target for osteoporosis.
Main Methods:
- Studied RILP expression in mouse bone marrow macrophages stimulated with RANKL.
- Utilized siRNA to inhibit RILP expression and assessed osteoclast characteristics.
- Analyzed osteoclast migration, F-actin ring formation, and cathepsin K secretion.
- Evaluated RILP inhibition in LPS-induced bone loss models.
Main Results:
- RILP expression is induced by RANKL in macrophages.
- RILP inhibition significantly decreased osteoclast number, size, and F-actin ring formation.
- RILP suppression impaired preosteoclast migration via PI3K-Akt signaling.
- Inhibition of RILP reduced cathepsin K secretion and bone resorption.
- siRNA-mediated RILP knockdown attenuated LPS-induced bone loss.
Conclusions:
- RILP is essential for osteoclast formation and bone resorption.
- RILP represents a promising therapeutic target for osteoporosis and related bone diseases.
Related Concept Videos
01:31Osteoclasts in Bone Remodeling
3.1K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
3.1K
01:40Bone Remodeling
38.4K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
38.4K
01:25Role of Vitamins in Maintaining Bone Health
3.5K
The growth and maintenance of bone are regulated by a combination of nutritional factors, including vitamins, such as vitamin A, B12, C, D, and K.
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...
3.5K
01:17Hormones and Bone Tissue
2.8K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
2.8K
01:31Regulation of the Unfolded Protein Response
2.5K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.5K
01:21Skeleton and Calcium Homeostasis
4.6K
Calcium is not only the most abundant mineral in bone but also the most abundant mineral in the human body. Calcium ions are needed for bone mineralization, tooth health, heart rate regulation and strength of contraction, blood coagulation, the contraction of smooth and skeletal muscle cells, and the regulation of nerve impulse conduction. The average calcium level in the blood is about 10 mg/dL. When the body cannot maintain this level, a person will experience hypo or hypercalcemia.
4.6K