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Evaluation of red-complex bacteria loads in complete denture patients: a pilot study
Enis Veseli1, Gloria Staka2,3, Marcos Roberto Tovani-Palone4
1Department of Prosthodontics, Dental School, Faculty of Medicine, University of Pristina, Pristina, Kosovo. enis.veseli@uni-pr.edu.
Objective:
This pilot study aimed to evaluate red-complex bacteria (RCB) loads in edentulous patients, before and after dentures' insertion.
Materials And Methods:
Thirty patients were included in the study. Deoxyribonucleic acid (DNA) isolated from bacterial samples were obtained from the dorsum of the tongue before and 3 months after complete dentures (CDs) insertion in order to identify the presence of RCB (Tannerella forsythia, Porphyromonas gingivalis, and Treponema denticola) and quantify their loads, using real-time polymerase chain reaction (RT-PCR). Bacterial loads were represented as "Lg (genome equivalents/sample)" and the data classified according to the "ParodontoScreen" test.
Results:
Significant changes in bacterial loads were observed before and 3 months after the CDs insertion for: P. gingivalis (0.40 ± 0.90 vs 1.29 ± 1.64, p = 0.0007), T. forsythia (0.36 ±0.94 vs 0.87 ± 1.45, p = 0.005), and T. denticola (0.11 ± 0.41 vs 0.33 ± 0.75, p = 0.03). Before the CDs insertion, all patients had a normal bacterial prevalence range (100%) for all analyzed bacteria. Three months after the insertion, 2 (6.7%) of them had a moderate bacterial prevalence range for P. gingivalis, while 28 (93.3%) had a normal bacterial prevalence range.
Conclusion:
The use of CDs has a significant impact on increasing RCB loads in edentulous patients.
