Tubulin sequence region beta 155-174 is involved in binding exchangeable guanosine triphosphate

Assembly-competent microtubule protein was directly photoaffinity labeled with [alpha-32P]guanosine triphosphate by UV irradiation. The labeled tubulin was digested with trypsin. The radioactive fragments were isolated and sequenced, revealing beta-tubulin residues 155-174 to be the major labeled region. An antibody to a synthetic peptide comprising residues beta 154-165 inhibits GTP incorporation and tubulin polymerization.