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Updated: Aug 9, 2025
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Permanent Cerebral Vessel Occlusion via Double Ligature and Transection
Published on: July 21, 2013
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A novel histological occlusion classification for coiled aneurysms based on multiphoton microscopy.
Szatmary Zoltan1,2, Cortese Jonathan2,3, Mounier Jeremy2,4
1Neuroradiology Department, Limoges University, Dupuytren Hospital, Limoges, France.
Summary
Multiphoton microscopy offers a novel way to assess intracranial aneurysm healing after coiling. This technique provides a five-stage histological scale for evaluating treatment efficacy and understanding aneurysm repair processes.
Area of Science:
- Neuroendovascular therapy
- Biomedical imaging
- Histopathology
Background:
- Intracranial aneurysm (IA) coiling is a primary endovascular treatment, but recanalization is a frequent complication.
- Angiographic occlusion does not equate to complete aneurysm healing, necessitating advanced evaluation methods.
- Histological assessment of embolized aneurysms is challenging.
Purpose of the Study:
- To analyze the coil healing process in intracranial aneurysms using multiphoton microscopy (MPM).
- To compare MPM with conventional histological staining for evaluating aneurysm healing.
- To establish a histological scale for assessing treatment success after IA coiling.
Main Methods:
- A rabbit elastase model was used to create 27 intracranial aneurysms.
- Aneurysms were treated with coil embolization and analyzed 1 month post-implantation.
- Histological sections were stained with Hematoxylin and Eosin (H&S) and imaged using MPM (autofluorescence and second-harmonic generation) to create 3D reconstructions.
Main Results:
- Multiphoton microscopy, combined with conventional histology, provided enhanced contrast for evaluating aneurysm healing.
- A novel five-level histological scale was developed based on thrombus evolution and extracellular matrix (ECM) deposition.
- This scale allows for a more precise assessment of healing stages.
Conclusions:
- A new histological scale for evaluating intracranial aneurysm healing after coiling was established using a rabbit model and nonlinear microscopy.
- This five-stage classification provides a precise tool for assessing the efficacy of occlusion devices.
- The study highlights the potential of innovative microscopy techniques for research in neuroendovascular treatments.
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