Disposable-micropipette tip supported electrified liquid-organogel interface as a platform for sensing acetylcholine
S Sudalaimani1,2, S Arun1, A Esokkiya1,2
1Electrodics and Electrocatalysis Division, CSIR-Central Electrochemical Research Institute, Karaikudi-630 003, Tamil Nadu, India. giribabuk@cecri.res.in.
The Analyst
|February 22, 2023
Summary
Researchers developed a novel liquid-organogel interface for sensing acetylcholine, offering a non-enzymatic electrochemical detection method. This new approach provides a sensitive and reliable way to detect acetylcholine in various applications.
Area of Science:
- Electrochemistry
- Biosensing
- Interface Science
Background:
- Acetylcholine detection traditionally relies on enzymatic methods due to its electrochemical inertness.
- Electrified liquid-liquid interfaces offer a pathway for detecting non-redox molecules beyond conventional solid electrode limitations.
Purpose of the Study:
- To develop a disposable micropipette tip-based platform for sensing acetylcholine using liquid-organogel and liquid-liquid interfaces.
- To evaluate the performance of these interfaces for acetylcholine detection, focusing on linearity, detection limits, and sensitivity.
Main Methods:
- Fabrication of a liquid-organogel interface on a disposable micropipette tip.
- Exploration of a liquid-liquid interface using a pre-pulled glass micropipette for acetylcholine sensing.
- Electrochemical measurements to analyze ion transfer across the interfaces.
Main Results:
- Both liquid-organogel and liquid-liquid interfaces showed a linear increase in current with acetylcholine concentration during backward transfer.
- The liquid-organogel interface demonstrated detection limits of 0.18 μM and 0.23 μM with sensitivities of 9.52 nA μM⁻¹ and 9.20 nA μM⁻¹.
- The liquid-liquid interface achieved detection limits of 0.42 μM and 0.13 μM with sensitivities of 5 × 10⁻³ nA μM⁻¹ and 3.39 × 10⁻² nA μM⁻¹.
Conclusions:
- The disposable micropipette tip-based liquid-organogel interface is highly suitable for electrified soft interface sensing applications.
- This non-enzymatic approach offers a promising alternative for acetylcholine detection.
- The developed interfaces provide sensitive and reliable electrochemical sensing of acetylcholine.
