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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
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Multiple flow cytometry analysis for assessing human sperm functional characteristics.

Josiane de Lima Rosa1, Camila de Paula Freitas Dell'Aqua2, Fabiana Ferreira de Souza2

  • 1Institute of Biosciences, Department of Structural and Functional Biology, Laboratory of Reproductive and Developmental Biology and Toxicology, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.

Reproductive Toxicology (Elmsford, N.Y.)
|February 22, 2023
PubMed
Summary

Multiparametric flow cytometry offers a precise method for sperm evaluation, assessing multiple sperm attributes simultaneously. This technique enhances the accuracy of male infertility diagnosis, crucial in the face of environmental toxicants.

Keywords:
Flow cytometryHuman spermMale infertilityMultiparametric analysisReproductive toxicity

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Area of Science:

  • Reproductive biology
  • Andrology
  • Flow cytometry

Background:

  • Sperm evaluation is critical for fertility assessment.
  • Current methods like microscopy have limitations in accuracy and cell number assessment.
  • Multiparametric analysis can improve precision in predicting fertility potential.

Purpose of the Study:

  • To develop novel multiparametric flow cytometry assays for sperm analysis.
  • To assess sperm plasma membrane integrity, acrosome status, mitochondrial potential, and superoxide anion production.
  • To validate these assays using human semen samples.

Main Methods:

  • Utilized flow cytometry with 2 and 3 lasers.
  • Analyzed human semen samples subjected to different treatments (frozen, mixed, fresh).
  • Employed fluorochrome-conjugated compounds for multiparametric staining.

Main Results:

  • Developed objective, sensitive, rapid, and reproducible flow cytometry protocols.
  • Demonstrated significant differences in stained sperm percentages across treatments.
  • Achieved determination coefficients close to 1, validating the methodologies.

Conclusions:

  • The developed flow cytometry methods accurately reflect the status of analyzed sperm structures.
  • This approach enables more precise diagnosis of male infertility.
  • The findings are significant given the increasing prevalence of infertility linked to environmental toxicants.