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Plasmid pMO1 from Marinitoga okinawensis, first non-cryptic plasmid reported within Thermotogota

Julien Lossouarn1, Camilla L Nesbø2, Nadège Bienvenu3

  • 1Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350, Jouy-en-Josas, France.

Research in Microbiology
|February 22, 2023
PubMed

Insights

Researchers identified a novel plasmid, pMO1, in deep-sea bacteria. This mobile genetic element likely influences its host

Area of Science:

  • Microbiology
  • Genetics
  • Extremophile Research

Background:

  • Mobile genetic elements (MGEs) like viruses and plasmids are key drivers of microbial evolution.
  • Understanding MGEs in extreme environments, such as deep-sea hydrothermal vents, is crucial but limited.
  • The phylum Thermotogota comprises extremophilic microorganisms inhabiting these unique ecosystems.

Purpose of the Study:

  • To investigate mobile genetic elements in deep-sea hydrothermal vent microorganisms.
  • To characterize a novel plasmid from the reference strain Marinitoga okinawensis.
  • To determine the potential functional role of this plasmid in its host.

Main Methods:

  • Isolation and sequencing of the plasmid pMO1 from Marinitoga okinawensis.
  • Assembly of the plasmid genome.
  • Functional gene annotation of the identified plasmid sequences.

Main Results:

  • Successfully isolated and sequenced a 28.2 kbp plasmid, designated pMO1.
  • Annotated pMO1, revealing genes for restriction/modification systems and chemotaxis proteins.
  • pMO1 is the first described non-cryptic plasmid within the phylum Thermotogota.

Conclusions:

  • The plasmid pMO1 likely impacts the phenotype of its host, Marinitoga okinawensis.
  • This discovery expands our knowledge of MGEs in extreme environments.
  • pMO1 provides insights into the evolutionary dynamics of Thermotogota.
Keywords:
Deep-sea hydrothermal ventPlasmidThermophilesThermotogota

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