Related Experiment Video

Updated: Aug 9, 2025

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

511

Differential effects of the LncRNA RNF157-AS1 on epithelial ovarian cancer cells through suppression of DIRAS3- and

Pengfei Xu1, Sujuan Xu2,3, Haiyue Pan2,4

  • 1Nanjing Maternity and Child Health Care Institute, Women's Hospital of Nanjing Medical University (Nanjing Maternity and Child Health Care Hospital), 210004, Nanjing, China. pengfeixu@njmu.edu.cn.

Cell Death & Disease
|February 22, 2023
PubMed

Insights

The long non-coding RNA RNF157-AS1 promotes epithelial ovarian cancer (EOC) progression and chemoresistance by inhibiting autophagy. Suppressing RNF157-AS1 may offer a therapeutic strategy for EOC patients undergoing chemotherapy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Epithelial ovarian cancer (EOC) is a leading cause of cancer-related deaths in women.
  • Long non-coding RNAs (lncRNAs) play critical roles in cancer development and progression.
  • RNF157 Antisense RNA 1 (RNF157-AS1) is implicated in various cancers, but its role in EOC remains unclear.

Purpose of the Study:

  • To investigate the role of RNF157-AS1 in EOC proliferation, invasion, and migration.
  • To elucidate the molecular mechanisms underlying RNF157-AS1's function in EOC.
  • To evaluate the potential of RNF157-AS1 as a therapeutic target in EOC chemotherapy.

Main Methods:

  • Database analysis and cell culture experiments to assess RNF157-AS1 expression and function.
  • RNA pulldown, RIP, mass spectrometry, and ChIP assays to identify RNF157-AS1 interacting proteins and binding sites.
  • Western blot, immunofluorescence, and CCK8 assays to analyze protein expression, autophagy levels, and chemosensitivity.

Main Results:

  • RNF157-AS1 is overexpressed in EOC tissues and promotes EOC cell proliferation, invasion, and migration.
  • RNF157-AS1 interacts with HMGA1 and EZH2 to suppress ULK1 and DIRAS3 expression, respectively, thereby inhibiting autophagy.
  • RNF157-AS1 overexpression enhances EOC cell sensitivity to cisplatin (DDP) chemotherapy by suppressing autophagy, leading to longer overall survival (OS) and progression-free survival (PPS).

Conclusions:

  • RNF157-AS1 acts as an oncogenic lncRNA in EOC by inhibiting autophagy.
  • RNF157-AS1-mediated suppression of autophagy enhances EOC cell sensitivity to DDP chemotherapy.
  • RNF157-AS1 represents a promising therapeutic target for improving chemotherapy outcomes in EOC patients.

More Related Videos

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer09:08

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

6.8K
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.7K

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.7K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K
The Nucleolus02:55

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
8.9K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K