Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Nucleosome Remodeling

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...

The Nucleosome

The Nucleosome

The Nucleosome Core Particle

The Nucleosome Core Particle

Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Minimal Genome, Maximal Effect: How Polyomavirus Genomes Are Capable of Complex Pathogenesis.

Viruses·2026

Same author

Baloxavir alleviates severe disease and viremia in ferrets infected with avian- or bovine-origin influenza A(H5N1) virus.

Science translational medicine·2026

Same author

Dysregulation of histone methylation in SV40 chromosomes during replication results in aberrant transcription and chromatin structure.

Tumour virus research·2025

Same author

Early in an SV40 infection, histone modifications correlate with the presence or absence of RNAPII and direction of transcription.

Virology·2022

Same author

SMAP is a pipeline for sample matching in proteogenomics.

Nature communications·2022

Same author

Comparing a new method for mapping nucleosomes in simian virus 40 chromatin to standard procedures.

Epigenetics·2020

Search research articles

Related Experiment Video

Updated: Aug 9, 2025

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Published on: September 10, 2013

Mapping Nucleosome Location Using FS-Seq.

Barry Milavetz¹, Brenna Hanson², Kincaid Rowbotham²

¹Department of Biomedical Sciences, School of Medicine, University of North Dakota, Grand Forks, ND, USA. barry.milavetz@UND.edu.

Methods in Molecular Biology (Clifton, N.J.)

|February 22, 2023

Summary

This summary is machine-generated.

Mapping nucleosome locations in chromatin is crucial for understanding gene regulation. This study details a new method using a specific kit for chromatin fragmentation, improving next-generation sequencing accuracy for nucleosome mapping.

Keywords:

Chromatin NGS Nucleosomes Phasing Sequencing

More Related Videos

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Published on: September 6, 2024

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

Published on: December 12, 2017

Related Experiment Videos

Last Updated: Aug 9, 2025

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Published on: September 10, 2013

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Published on: September 6, 2024

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

Published on: December 12, 2017

Area of Science:

Molecular Biology
Genetics
Epigenetics

Background:

Nucleosome organization in eukaryotic chromatin is vital for regulating biological functions.
Accurate mapping of nucleosome positions is essential for understanding gene expression and chromatin dynamics.
Existing techniques for nucleosome mapping involve chromatin fragmentation followed by next-generation sequencing.

Purpose of the Study:

To describe and evaluate a novel procedure for chromatin fragmentation using the NEB NEXT Ultra II FS DNA library prep Kit.
To compare this new fragmentation method with other available procedures for mapping nucleosome location.
To assess the utility of this kit for precise nucleosome positioning analysis.

Main Methods:

Utilized the NEB NEXT Ultra II FS DNA library prep Kit for chromatin fragmentation.
Employed next-generation sequencing to analyze the fragmented chromatin.
Compared the results with established methods for nucleosome mapping.

Main Results:

The NEB NEXT Ultra II FS DNA library prep Kit effectively fragments chromatin for nucleosome mapping.
This method offers a viable alternative for accurate nucleosome location determination.
Performance was evaluated against other standard procedures.

Conclusions:

The described procedure provides a reliable and efficient method for mapping nucleosome locations.
This technique aids in the study of chromatin organization and its regulatory roles.
The NEB kit presents a valuable tool for chromatin research and next-generation sequencing applications.