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Updated: Aug 9, 2025
Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
Published on: September 10, 2013
Mapping Nucleosome Location Using FS-Seq
Barry Milavetz1, Brenna Hanson2, Kincaid Rowbotham2
1Department of Biomedical Sciences, School of Medicine, University of North Dakota, Grand Forks, ND, USA. barry.milavetz@UND.edu.
Abstract:
The organization of nucleosomes in eukaryotic chromatin is thought to play a critical role in the regulation of the biological function of the chromatin. Because of this potential role in regulation, a number of techniques have been developed, which combine chromatin fragmentation around nucleosomes with next-generation sequencing to map the location of nucleosomes in chromatin. In this section, a procedure using a kit from New England Biolabs (NEB NEXT Ultra II FS DNA library prep Kit) to fragment chromatin in preparation for next-generation sequencing is described and compared to other available procedures for mapping nucleosome location.
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