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Related Concept Videos

Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

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The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
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Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
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Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
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Related Experiment Video

Updated: Aug 9, 2025

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
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Profiling Chromatin Accessibility on Replicated DNA with repli-ATAC-Seq.

Kathleen R Stewart-Morgan1,2, Anja Groth3,4

  • 1Novo Nordisk Foundation Center for Protein Research (CPR), Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed repli-ATAC-seq to study accessible chromatin after DNA replication. This new method profiles chromatin dynamics, revealing crucial insights into how replication impacts transcription and the overall chromatin landscape.

Keywords:
Accessible chromatinChromatin assemblyDNA replicationNucleosome-free regionsTranscriptionrepli-ATAC-seq

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Area of Science:

  • Molecular Biology
  • Genomics
  • Epigenetics

Background:

  • Open chromatin defines cell-specific transcription, but its response to DNA replication is unclear.
  • DNA replication significantly disrupts chromatin structure and composition.
  • Understanding chromatin recovery post-replication is vital for cell biology.

Purpose of the Study:

  • To develop a novel method for profiling accessible chromatin on replicated DNA.
  • To investigate the dynamics of chromatin accessibility following DNA replication.
  • To elucidate the interplay between DNA replication, transcription, and chromatin.

Main Methods:

  • Introduced repli-ATAC-seq, a protocol for genome-wide chromatin accessibility profiling.
  • Utilized 5-ethynyl-2'-deoxyuridine (EdU) pulse-labeling of replicated DNA in cultured cells.
  • Isolated and sequenced tagmented fragments from replicated DNA, starting with 100,000 cells.

Main Results:

  • Repli-ATAC-seq provides high-resolution data on chromatin dynamics post-DNA replication.
  • The method enables detailed analysis of accessible chromatin regions after replication.
  • New insights into the relationship between DNA replication and the chromatin landscape were uncovered.

Conclusions:

  • Repli-ATAC-seq is an effective tool for studying chromatin accessibility dynamics.
  • The study sheds light on how cells manage chromatin structure during and after replication.
  • Findings contribute to understanding the regulation of transcription and cell identity.