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Updated: Aug 9, 2025
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Purification of Mitochondria from Yeast Cells
Published on: August 24, 2009
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Isolation and Quality Control of Yeast Mitochondria
Asli Aras Taskin1,2, Daiana Nerina Moretti1,3, F Nora Vögtle2,4
1Institute of Biochemistry and Molecular Biology, ZBMZ, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2023
Abstract:
The isolation of organelles devoid of other cellular compartments is crucial for studying organellar proteomes and the localization of newly identified proteins, as well as for assessing specific organellar functions. Here, we describe a protocol for the isolation of crude and highly pure mitochondria from Saccharomyces cerevisiae and provide methods for testing the functional integrity of the isolated organelles.
