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In Cellulo Light-Induced Dynamics in a BODIPY-Perylene Dyad
Tingxiang Yang1,2, Ruben Arturo Arellano-Reyes3, Rhianne C Curley3
1Leibniz Institute of Photonic Technology (Leibniz-IPHT), Albert-Einstein-Street 9, 07745, Jena, Germany.
Chemistry (Weinheim an Der Bergstrasse, Germany)
|February 22, 2023
Summary
This study investigates the excited-state dynamics of a BODIPY-perylene dyad, a sensor for live cell viscosity. Understanding these properties in cellulo is crucial for developing advanced biological probes.
Area of Science:
- Photophysics
- Biophysical Chemistry
- Chemical Biology
Background:
- BODIPY-donor-acceptor dyads are vital fluorescent probes in life sciences.
- Their behavior in solution is well-understood, but in-cellulo photophysics remain less characterized.
- Accurate characterization in cellular environments is essential for probe design.
Purpose of the Study:
- To investigate the excited-state dynamics of a BODIPY-perylene dyad.
- To understand the photophysical properties of this dyad within a cellular environment.
- To validate its function as a probe for local viscosity in live cells.
Main Methods:
- Sub-nanosecond time-resolved transient absorption spectroscopy.
- Study of a BODIPY-perylene dyad designed for viscosity sensing.
- In cellulo measurements to assess photophysical properties.
Main Results:
- Detailed analysis of excited-state dynamics was performed.
- The study provides insights into the dyad's behavior in a cellular context.
- The dyad's potential as a viscosity probe was evaluated.
Conclusions:
- The excited-state dynamics of the BODIPY-perylene dyad were elucidated.
- This research bridges the gap between in-solution and in-cellulo understanding of dye behavior.
- Findings support the use of such dyads as probes for live-cell biophysical parameters.
