Size-dependent response of cells in epithelial tissue modulated by contractile stress fibers

Although cells with distinct apical areas have been widely observed in epithelial tissues, how the size of cells affects their behavior during tissue deformation and morphogenesis as well as key physical factors modulating such influence remains elusive. Here, we showed that the elongation of cells within the monolayer under anisotropic biaxial stretching increases with their size because the strain released by local cell rearrangement (i.e., T1 transition) is more significant for small cells that possess higher contractility. On the other hand, by incorporating the nucleation, peeling, merging, and breakage dynamics of subcellular stress fibers into classical vertex formulation, we found that stress fibers with orientations predominantly aligned with the main stretching direction will be formed at tricellular junctions, in good agreement with recent experiments. The contractile forces generated by stress fibers help cells to resist imposed stretching, reduce the occurrence of T1 transitions, and, consequently, modulate their size-dependent elongation. Our findings demonstrate that epithelial cells could utilize their size and internal structure to regulate their physical and related biological behaviors. The theoretical framework proposed here can also be extended to investigate the roles of cell geometry and intracellular contraction in processes such as collective cell migration and embryo development.