Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multimodal imaging of gene expression, morphology, and activity of the same neuron.

Cell·2026
Same author

Rbm24 promotes outer hair cell survival through Insm1 repression while independently regulating hair bundle morphogenesis.

Development (Cambridge, England)·2026
Same author

Functional heterogeneity of medial septal GABAergic neurons in theta rhythm modulation and septo-hippocampal communication.

Brain research bulletin·2026
Same author

Evolutionary convergence and divergence of hippocampal cytoarchitecture between rodents and primates revealed by single-cell spatial transcriptomics.

National science review·2026
Same author

Letter to the Editor on "A novel algorithm-based risk classification for vascular damage in men with erectile dysfunction".

The journal of sexual medicine·2026
Same author

Evaluating the predictors of poor corporal integrity in penile implant recipients.

BJU international·2025

Related Experiment Video

Updated: Aug 9, 2025

Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons
11:21

Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons

Published on: November 20, 2018

8.5K

Calbindin-Expressing CA1 Pyramidal Neurons Encode Spatial Information More Efficiently.

Liqin Gu1, Minglong Ren1, Longnian Lin2,3,4

  • 1Institute of Brain Functional Genomics, East China Normal University, Shanghai 200062, China.

Eneuro
|February 22, 2023
PubMed
Summary

Calbindin-positive (CB+) hippocampal neurons represent spatial information more efficiently than CB- neurons. This study reveals functional heterogeneity in hippocampal pyramidal neurons during spatial tasks and sleep.

Keywords:
firing patternhippocampuspyramidal neuronripplespatial codingtheta

More Related Videos

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices
10:35

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Published on: March 15, 2018

10.9K
Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons
10:29

Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons

Published on: October 8, 2014

14.1K

Related Experiment Videos

Last Updated: Aug 9, 2025

Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons
11:21

Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons

Published on: November 20, 2018

8.5K
Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices
10:35

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Published on: March 15, 2018

10.9K
Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons
10:29

Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons

Published on: October 8, 2014

14.1K

Area of Science:

  • Neuroscience
  • Cellular Neuroscience
  • Systems Neuroscience

Background:

  • Hippocampal pyramidal neurons (PNs) were traditionally viewed as a uniform population.
  • Recent findings highlight significant structural and functional heterogeneity within hippocampal PNs.
  • Neuronal firing patterns of molecularly identified PN subclasses in vivo remain largely uncharacterized.

Purpose of the Study:

  • To investigate the in vivo firing patterns of hippocampal PNs based on Calbindin (CB) expression.
  • To explore the role of CB+ and CB- PNs in spatial information representation during a spatial shuttle task.
  • To examine the differential involvement of CB+ and CB- PNs in sleep-related oscillations.

Main Methods:

  • Utilized a spatial shuttle task in freely moving male mice.
  • Recorded neuronal activity and analyzed firing patterns of Calbindin-positive (CB+) and Calbindin-negative (CB-) pyramidal neurons.
  • Investigated neuronal firing phase shifts during Rapid-Eye Movement (REM) sleep and running epochs.
  • Assessed modulation of neuronal activity during ripple oscillations in Slow-Wave Sleep (SWS).

Main Results:

  • CB+ place cells demonstrated more efficient spatial information representation compared to CB- place cells, despite lower firing rates during running.
  • A subset of CB+ PNs exhibited altered theta firing phase during REM sleep relative to running states.
  • CB- PNs were more involved in ripple oscillations, whereas CB+ PNs showed stronger modulation during SWS ripples.
  • Evidence suggests potential enhanced input from the lateral entorhinal cortex to CB+ PNs.

Conclusions:

  • The study reveals significant functional heterogeneity between hippocampal CB+ and CB- PNs.
  • CB+ PNs contribute to more efficient spatial encoding, possibly due to specific afferent connections.
  • These findings challenge the homogeneous view of hippocampal pyramidal neurons and highlight subclass-specific roles in cognition and memory.