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Expression of a pseudogene for interferon-alpha L.

X Y Liu¹, V Jung, X X Zhao

¹Shanghai Institute of Biochemistry, Academia Sinica, China.

Journal of Interferon Research

|October 1, 1987

Summary

This summary is machine-generated.

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Human leukocyte interferon-alpha L (IFN-alpha L), initially thought to be a pseudogene, was tested for activity. Researchers found that IFN-alpha L is biologically active on human and bovine cells, despite a DNA termination codon.

Area of Science:

Molecular Biology
Immunology
Genetics

Background:

Human leukocyte interferon-alpha L (IFN-alpha L) possesses a DNA termination codon within its precursor peptide sequence.
Despite this, the gene encodes an interferon protein of normal length, raising questions about its functional status.

Purpose of the Study:

To investigate the biological activity of the human leukocyte interferon-alpha L (IFN-alpha L) gene.
To determine if the IFN-alpha L protein is functional despite a premature DNA termination codon.

Main Methods:

Construction of an expression plasmid for IFN-alpha L.
Production of the IFN-alpha L protein in Escherichia coli.
Assay of the expressed IFN-alpha L's activity on various cell types.

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Main Results:

The expressed IFN-alpha L demonstrated significant biological activity on human and bovine cells.
A trace level of activity was also observed on mouse cells.

Conclusions:

The human leukocyte interferon-alpha L (IFN-alpha L) gene codes for a biologically active interferon molecule.
The termination codon does not fully abrogate the functional capacity of the IFN-alpha L protein.