Long noncoding RNA 02027 inhibits proliferation, migration and invasion of hepatocellular carcinoma via
Jinyi Wang1,2, Yong Zhu3, Xiaoming Ai4
1Hepatobiliary Center, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Background:
Long non-coding RNAs have been established to promote or inhibit the oncogenic and tumorigenic potential of various cancers, acting as competing endogenous RNAs (ceRNAs) for specific microRNAs. The primary objective of the study was to investigate the underlying mechanism by which the LINC02027/miR-625-3p/PDLIM5 axis affects proliferation, migration and invasion in hepatocellular carcinoma (HCC).
Methods:
The differentially expressed gene was selected based on gene sequencing and bioinformation database analysis of HCC and adjacent non-tumor tissues. The expression of LINC02027 in HCC tissues and cells and its regulatory effect on the development of HCC were detected by colony formation, cell counting kit-8 assays, wound healing assays, Transwell assays and subcutaneous tumorigenesis assays in nude mice. According to the results of database prediction, quantitative real-time polymerase chain reaction and dual-luciferase reporter assay, the downstream microRNA and target gene were searched. Finally, HCC cells were transfected with lentivirus and used for cell function assays in vitro and in vivo.
Results:
Downregulation of LINC02027 was detected in HCC tissues and cell lines and was associated with poor prognosis. The overexpression of LINC02027 suppressed the proliferation, migration and invasion of HCC cells. Mechanistically, LINC02027 inhibited epithelial-to-mesenchymal transition. As a ceRNA, LINC02027 inhibited the malignant ability of HCC by competitively binding to miR-625-3p to regulate the expression of PDLIM5.
Conclusions:
The LINC02027/miR-625-3p/PDLIM5 axis inhibits the development of HCC.
Insights
Long non-coding RNA LINC02027 suppresses hepatocellular carcinoma (HCC) progression by inhibiting proliferation, migration, and invasion. This involves the LINC02027/miR-625-3p/PDLIM5 axis acting as a competing endogenous RNA network.
Area of Science:
- Molecular oncology
- Cancer biology
- RNA biology
Background:
- Long non-coding RNAs (lncRNAs) modulate cancer development by acting as competing endogenous RNAs (ceRNAs).
- Understanding lncRNA roles in hepatocellular carcinoma (HCC) is crucial for targeted therapies.
Purpose of the Study:
- To elucidate the mechanism of the LINC02027/miR-625-3p/PDLIM5 axis in HCC proliferation, migration, and invasion.
Main Methods:
- Gene sequencing and bioinformatics analysis identified differentially expressed genes in HCC.
- In vitro and in vivo assays assessed LINC02027 expression and function in HCC.
- Quantitative real-time PCR and dual-luciferase reporter assays identified the miR-625-3p and PDLIM5 interaction.
Main Results:
- LINC02027 was downregulated in HCC tissues and associated with poor prognosis.
- Overexpression of LINC02027 inhibited HCC cell proliferation, migration, invasion, and epithelial-to-mesenchymal transition.
- LINC02027 acted as a ceRNA, competitively binding miR-625-3p to regulate PDLIM5 expression.
Conclusions:
- The LINC02027/miR-625-3p/PDLIM5 axis functions as a tumor suppressor in HCC.
- Targeting this axis may offer a novel therapeutic strategy for hepatocellular carcinoma.
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