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G-quadruplex-hemin DNAzyme functionalized nanopipettes: Fabrication and sensing application
Jingyi Dong1, Xia Qiu1, Mimi Huang1
1Key Laboratory of Functional Molecular Solids (Ministry of Education), Anhui Key Laboratory of Chemo/Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, PR China.
Talanta
|February 22, 2023
Summary
This study presents a new hydrogen peroxide sensor using gold-coated nanopipettes functionalized with G-quadruplex-hemin DNAzyme (GQH). The sensor monitors ion current changes for sensitive H2O2 detection in confined environments.
Area of Science:
- Nanotechnology
- Biochemistry
- Analytical Chemistry
Background:
- Solid-nanopores and nanopipettes offer advantages like adjustable size, rigidity, and low noise for molecular volume change detection.
- G-quadruplex-hemin DNAzyme (GQH) is a catalytically active DNA structure with potential in sensing applications.
Purpose of the Study:
- To establish a novel sensing platform using GQH-functionalized gold-coated nanopipettes.
- To develop a method for real-time monitoring of enzymatic catalysis in a confined nanopipette environment.
- To enable sensitive detection of hydrogen peroxide (H2O2).
Main Methods:
- Immobilization of G-quadruplex-hemin DNAzyme (GQH) onto gold-coated nanopipettes.
- Utilizing GQH as a catalyst for the reaction between H2O2 and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt).
- Monitoring real-time changes in transmembrane ion current to quantify H2O2 concentration.
Main Results:
- A correlation was established between ion current and H2O2 concentration within a specific range under optimal conditions.
- The GQH-functionalized nanopipette demonstrated effective hydrogen peroxide sensing.
- The system allowed for real-time monitoring of enzymatic catalysis within the confined nanopipette space.
Conclusions:
- The GQH-immobilized nanopipette serves as a versatile platform for studying enzymatic catalysis in confined environments.
- This approach is applicable to electrocatalysis, sensing, and fundamental electrochemistry research.
- The developed method offers a sensitive and real-time approach for hydrogen peroxide detection.
