Solid-state fermentation-based enzyme-assisted extraction of eicosapentaenoic acid-rich oil from Nannochloropsis sp

Enzymatic treatment of microalgal biomass is a promising approach for extraction of microalgal lipid, but high cost of commercially sourcing enzyme is a major drawback in industrial implementation. Present study involves extraction of eicosapentaenoic acid-rich oil from Nannochloropsis sp. biomass using low cost cellulolytic enzymes produced from Trichoderma reesei in a solid-state fermentation bioreactor. Maximum total fatty acid recovery of 369.4 ± 4.6 mg/g dry weight (total fatty acid yield of 77%) was achieved in 12 h from the enzymatically treated microalgal cells, of which the eicosapentaenoic acid content was 11%. Sugar release of 1.70 ± 0.05 g/L was obtained post enzymatic treatment at 50 °C. The enzyme was reused thrice for cell wall disruption without compromising on total fatty acid yield. Additionally, high protein content of 47% in the defatted biomass could be explored as a potential aquafeed, thus enhancing the overall economics and sustainability of the process.