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Developmental function of Piezo1 in mouse submandibular gland morphogenesis.

Elina Pokharel1, Yam Prasad Aryal1, Tae-Young Kim1

  • 1Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, 41940, South Korea.

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|February 22, 2023
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Summary

Piezo1, a mechanosensitive channel, is crucial for mouse salivary gland development. This study reveals Piezo1 regulates acinar cell differentiation by modulating the Shh signaling pathway.

Keywords:
Acinar cellExpression patternOrganogenesisSalivary gland developmentSignaling modulations

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Physiology

Background:

  • Mechanical forces are vital for organogenesis, particularly in secretory gland formation.
  • Piezo-type mechanosensitive ion channel component 1 (Piezo1) has been studied for mechanotransduction but its developmental role is less understood.

Purpose of the Study:

  • To investigate the developmental function of Piezo1 in mouse submandibular gland (SMG) development.
  • To analyze Piezo1's expression and localization during critical SMG development stages.

Main Methods:

  • Immunohistochemistry and RT-qPCR were used to analyze Piezo1 localization and expression in developing mouse SMGs.
  • siRNA-mediated knockdown of Piezo1 was performed during in vitro organ cultivation of E14 SMGs.
  • Histomorphology and expression of signaling molecules (Bmp2, Fgf4, Fgf10, Gli1, Gli3, Ptch1, Shh, Tgfβ-3, Aquaporin5, E-cadherin, Vimentin, cytokeratins) were examined.

Main Results:

  • Piezo1 was specifically expressed in acinar-forming epithelial cells during key differentiation stages (E14 and E16).
  • Loss of Piezo1 function altered SMG histomorphology and the expression patterns of key signaling molecules.
  • Altered localization of differentiation markers (Aquaporin5, E-cadherin, Vimentin, cytokeratins) was observed.

Conclusions:

  • Piezo1 plays a significant role in the early differentiation of salivary gland acinar cells.
  • Piezo1 appears to modulate acinar cell differentiation through the Shh signaling pathway.