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bioRxiv : the preprint server for biology·2026
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Related Experiment Video

Updated: Aug 9, 2025

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Solid-phase microextraction of endogenous metabolites from intact tissue validated using a Biocrates standard

Runshan Will Jiang1, Karol Jaroch1,2, Janusz Pawliszyn1

  • 1Department of Chemistry, University of Waterloo, Waterloo, N2L 3G1, Canada.

Journal of Pharmaceutical Analysis
|February 23, 2023
PubMed
Summary

Solid-phase microextraction (SPME) offers a minimally invasive method for tissue metabolomics, preserving the endogenous metabolome. This technique avoids disruptive homogenization, revealing distinct metabolite profiles compared to conventional methods.

Keywords:
In vivo samplingMetabolomicsSample preparationSolid-phase microextractionSolvent extraction

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Area of Science:

  • Metabolomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Conventional tissue metabolomics relies on homogenization, which alters the endogenous metabolome.
  • There is a need for improved analytical methods that preserve sample integrity.

Purpose of the Study:

  • To evaluate solid-phase microextraction (SPME) for minimally invasive tissue metabolomics.
  • To compare metabolite profiles from SPME with conventional methods on homogenized and intact ovine lung tissue.

Main Methods:

  • Solid-phase microextraction (SPME) was used on homogenized and intact ovine lung tissue.
  • Downstream targeted metabolomics analysis was performed using a Biocrates AbsoluteIDQ assay.
  • Statistical analysis, including principal component analysis, was employed to compare sample groups.

Main Results:

  • SPME revealed significant differences in metabolite profiles between homogenized and intact tissue.
  • Conventional liquid extraction from homogenized tissue differed from SPME results.
  • Principal component analysis showed distinct clustering for homogenized, intact tissue (SPME), and conventional extraction groups.

Conclusions:

  • Tissue homogenization significantly alters the endogenous metabolome.
  • SPME is a valuable tool for minimally invasive tissue metabolomics, preserving metabolite integrity.
  • SPME provides broad metabolite coverage without the need for tissue biopsy or homogenization.