Related Experiment Video
Updated: Aug 9, 2025
05:39
Murine Model of Wound Healing
Published on: May 28, 2013
64.8K
Protocol for the Splinted, Human-like Excisional Wound Model in Mice.
Katharina S Fischer1, Ben Litmanovich1, Dharshan Sivaraj1,2
1Department of Surgery, University of Arizona College of Medicine, Tucson, AZ, 85724, USA.
Bio-Protocol
|February 23, 2023
Summary
The splinted excisional wound model in mice overcomes rodent-specific wound contraction, enabling more accurate study of human-like wound healing. This refined protocol ensures reproducible and valid results for research.
Area of Science:
- Biomedical research
- Regenerative medicine
- Animal models in research
Background:
- Human wound healing primarily involves re-epithelialization.
- Rodent models exhibit panniculus carnosus muscle contraction, complicating wound healing studies.
- Murine models are cost-effective and versatile for wound healing research.
Purpose of the Study:
- To present an updated protocol for the splinted excisional wound model in mice.
- To refine techniques for generating valid and reproducible wound healing data.
- To provide a standardized method for studying human-like wound healing in rodents.
Main Methods:
- Utilizing the established splinted excisional wound model in mice.
- Implementing refined surgical and data collection techniques.
- Applying standardized data analysis methods for wound healing kinetics.
Main Results:
- The splinted excisional wound model effectively minimizes wound contraction bias.
- The refined protocol yields highly valid and reproducible experimental results.
- The model facilitates accurate analysis of wound healing processes.
Conclusions:
- The splinted excisional wound model offers a simple, standardizable, and replicable approach to studying human-like wound healing.
- This refined protocol enhances the reliability of murine models for wound healing research.
- The model is crucial for advancing our understanding of wound repair mechanisms.
