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Published on: May 27, 2016
Circulating exosomal lncRNAs in patients with chronic coronary syndromes
Meili Zheng1,2, Ruijuan Han3, Wen Yuan4
1Heart Center, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.
Insights
Exosomal lncRNAs ENST00000424615.2 and ENST00000560769.1 show diagnostic potential for chronic coronary syndrome (CCS). ENST00000560769.1 may indicate disease severity in CCS patients.
Area of Science:
- Cardiovascular Research
- Molecular Biology
- Biomarker Discovery
Background:
- Chronic coronary syndrome (CCS) is a clinical concept introduced in 2019.
- The role of exosomal long non-coding RNAs (lncRNAs) in CCS pathogenesis and diagnosis is not well understood.
Purpose of the Study:
- To investigate the potential of exosomal lncRNAs as diagnostic biomarkers for CCS.
- To identify specific exosomal lncRNAs differentially expressed in CCS patients.
Main Methods:
- A case-control study involving 218 participants (15 CCS patients and 15 controls for sequencing; 20+20 for first validation; 100+48 for second validation).
- Exosomes were isolated from plasma, and exosomal lncRNAs were identified via sequencing and validated using qRT-PCR.
- Receiver operating characteristic (ROC) curve analysis was employed to assess diagnostic accuracy.
Main Results:
- 152 differentially expressed lncRNAs were identified in CCS patients' plasma exosomes.
- Exosomal lncRNAs ENST00000424615.2 and ENST00000560769.1 were significantly upregulated in CCS patients.
- ROC analysis yielded areas under the curve of 0.654 for ENST00000424615.2 and 0.722 for ENST00000560769.1.
- ENST00000560769.1 levels correlated with the number of diseased vessels (p=0.028).
Conclusions:
- Exosomal lncRNAs ENST00000424615.2 and ENST00000560769.1 are potential novel diagnostic biomarkers for CCS.
- ENST00000560769.1 may serve as a biomarker for disease severity and potentially poor prognosis in CCS.
Introduction:
The concept of chronic coronary syndrome (CCS) was first presented at the European Society of Cardiology Meeting in 2019. However, the roles of exosomal lncRNAs in CCS remain largely unclear.
Material And Methods:
A case-control study was performed with a total of 218 participants (137 males and 81 females), including 15 CCS patients and 15 controls for sequencing profiles, 20 CCS patients and 20 controls for the first validation, and 100 CCS patients and 48 controls for the second validation. Exosomes were isolated from the plasma of CCS patients and controls, and exosomal lncRNAs were identified by sequencing profiles and verified twice by qRT-PCR analysis. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic value of exosomal lncRNAs for CCS patients.
Results:
A total of 152 significantly differentially expressed lncRNAs with over two-fold changes were detected in plasma exosomes of CCS patients, including 90 upregulated and 62 downregulated lncRNAs. Importantly, 6 upregulated lncRNAs with the top fold changes were selected for validations. Exosomal lncRNAs ENST00000424615.2 and ENST00000560769.1 were significantly elevated in CCS patients in both validations compared with controls. The areas under the ROC of lncRNAs ENST00000424615.2 and ENST00000560769.1 were 0.654 and 0.722, respectively. Additionally, exosomal lncRNA ENST00000560769.1 was significantly higher in the CCS patients with more diseased vessels (p = 0.028).
Conclusions:
Exosomal lncRNA ENST00000424615.2 and ENST00000560769.1 were identified as novel diagnosis biomarkers for patients with CCS. Moreover, exosomal lncRNA ENST00000560769.1 was significantly higher in the CCS patients with more diseased vessels, and might be associated with a poor prognosis.
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