Related Experiment Video
Updated: Aug 9, 2025
Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
Published on: September 7, 2021
Extended application of BACs-on-Beads technique in prenatal diagnosis
Shiyu Sun1,2, Zhonghua Zhang3, Jing Zhao4
1Department of Medical Genetics, The Affiliated Weihai Second Municipal Hospital of Qingdao University (Weihai Maternity and Child Care Hospital), Weihai, China.
Introduction:
This study explored the application of bacterial artificial chromosomes (BACs)-on-Beads (BoBs) technique, especially its ability to detect microdeletion/microduplication regions with a single probe.
Methods:
Both chromosome karyotyping and BoBs technique were applied on a total of 2218 pregnant women. Chromosome microarray analysis (CMA) was performed on patients whose cells were reported as being abnormal by BoBs technique with a single probe.
Results:
Twenty-two cases were detected as microdeletion/microduplication with a single probe, which was consistent with the CMA results.
Conclusions:
We believe that the microdeletion/microduplication results detected by BoBs technique with a single probe provide comprehensive guidance for prenatal diagnosis.
