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Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels
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Temperature sensing by the calcium-sensing receptor.

Sarah C Brennan1,2, Hee-Chang Mun2, Leigh Delbridge3

  • 1School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Sydney, NSW, Australia.

Frontiers in Physiology
|February 23, 2023
PubMed
Summary
This summary is machine-generated.

The calcium-sensing receptor (CaSR) enables cells to sense temperature changes, influencing calcium signaling. This G protein-coupled receptor (GPCR) mechanism involves repetitive phosphorylation and dephosphorylation, suggesting broader roles in temperature sensing.

Keywords:
GPCRcalcium sensing receptorintracellular calcium oscillationsprotein kinase Cprotein phosphatasetemperature sensing

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Area of Science:

  • Cellular Biology
  • Neuroscience
  • Biochemistry

Background:

  • Extracellular calcium (Ca2+o) influences body temperature and neuronal activity.
  • The role of calcium-sensing receptors (CaSR) in temperature sensing is not well understood.
  • CaSRs are found in brain regions regulating temperature, and mutations are linked to seizures.

Purpose of the Study:

  • To investigate if CaSR-dependent signaling is sensitive to temperature.
  • To explore the mechanism by which CaSR might sense temperature.

Main Methods:

  • Utilized CaSR-expressing HEK-293 cells as a model system.
  • Analyzed Ca2+o-induced Ca2+i oscillations in response to temperature variations.
  • Employed chimeric receptor analysis and site-directed mutagenesis (T888A, T888M).
  • Used the PKC inhibitor GF109203X.

Main Results:

  • CaSR signaling frequency, not integrated response, showed linear temperature dependence within a relevant range.
  • The C-terminus of CaSR is crucial for temperature-dependent modulation.
  • Repetitive phosphorylation and dephosphorylation are vital for this temperature-sensing mechanism.

Conclusions:

  • CaSRs play a role in temperature sensing.
  • The mechanism involves CaSR phosphorylation and dephosphorylation.
  • This suggests G protein-coupled receptors (GPCRs) may generally contribute to thermosensation.