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circ_0000376 knockdown suppresses non-small cell lung cancer cell tumor properties by the miR-545-3p/PDPK1 pathway

Changpeng Sun1, Hongjun Guan2, Jinjin Li2

  • 1Department of Cardiothoracic Surgery, Jianhu Clinical Medical College of Yangzhou University, No. 666, Nanhuan Road, Jinhu Town, Jianhu, Yancheng City, Jiangsu Province, 224700, PR China.

Open Medicine (Warsaw, Poland)
|February 23, 2023
PubMed

Insights

Circular RNA circ_0000376 promotes non-small cell lung cancer (NSCLC) progression by regulating the miR-545-3p/PDPK1 pathway. Targeting circ_0000376 offers a potential therapeutic strategy for NSCLC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Non-small cell lung cancer (NSCLC) is a leading cause of cancer mortality worldwide.
  • Circular RNAs (circRNAs) are increasingly recognized for their roles in cancer development.
  • The specific function of circ_0000376 in NSCLC remains largely unexplored.

Purpose of the Study:

  • To investigate the role and mechanism of circ_0000376 in non-small cell lung cancer (NSCLC).
  • To explore the relationship between circ_0000376, miR-545-3p, and PDPK1 in NSCLC progression.

Main Methods:

  • Analysis of circ_0000376 and PDPK1 expression levels in NSCLC tissues and cells.
  • Assessment of miR-545-3p expression in NSCLC.
  • Investigation of the correlation between circ_0000376 expression and clinical parameters.
  • Functional assays including cell proliferation, migration, invasion, apoptosis, and glutaminolysis.
  • In vivo tumor formation experiments.

Main Results:

  • Circ_0000376 and PDPK1 expression were significantly upregulated, while miR-545-3p was downregulated in NSCLC.
  • Circ_0000376 expression correlated with lymph node metastasis, TNM stage, and tumor size.
  • Knockdown of circ_0000376 inhibited NSCLC cell proliferation, migration, invasion, and glutaminolysis, while inducing apoptosis.
  • Circ_0000376 acts as a sponge for miR-545-3p, which targets PDPK1, thereby regulating NSCLC phenotypes.
  • Circ_0000376 knockdown suppressed tumor growth in vivo.

Conclusions:

  • Circ_0000376 promotes NSCLC progression through the miR-545-3p/PDPK1 axis.
  • Circ_0000376 represents a potential diagnostic biomarker and therapeutic target for NSCLC.
Keywords:
NSCLCPDPK1circ_0000376miR-545-3p

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